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Mechancial Stimulation for cell culture made easy The TC-3 is a simple and multipurpose cell culture bioreactor designed to provide mechanical stimulation to a variety of samples and tissues. It permits to work with: Horizontally and vertically arranged multiple samples Immersed and air-liquid interface setups Sheet-shaped, rod-shaped and cynlinder-shaped substrates Tension and compssion axial loading Mechancial Stimulation for cell culture made easy 骨软骨肌腱韧带组织工程体外细胞组织拉伸压缩应力加载系统The TC-3 is a simple and multipurpose cell culture bioreactor designed to provide mechanical stimulation to a variety of samples and tissues. It permits to work with:•Horizontally and vertically arranged multiple samples•Immersed and air-liquid interface setups•Sheet-shaped, rod-shaped and cynlinder-shaped substrates•Tension and compssion axial loadingIntelligent designThe TC-3 bioreactor allows subjecting culture substrates to tension and compssion mechanical stimuli. It has been designed taking into account the needs of cell culture researchers, so only the features of tension machines which are basical for the culture of cells have be included in the TC-3.Among them, the possibility of inspecting the culture by microscopy techniques is of vital importance for the evaluation of culture status. A simple connection system between the culture chambers and the rest of the system has been designed. Thereby, the chamber can be easily removed from the system maintaining a desired deformation state while it is taken to the microscope for visual inspection of the culture.The TC-3 is compatible with most of the incubators, it is lightweight and all the parts in contact with the culture mediumcan sterilized by autoclaving.Main Features Click on image to enlargeVersatile chambers allow culture inspection by microscopy techniquesCulture chambers are made of autoclavable materials and can be easily assembled and disassebled. Furthermore, visual inspection of the chambers by microscopy techniques is allowed by a optical grade window glass at the bottom of the chamber.Three grips models adapting to your sample typeThree different models of interchangeable grips can be used with the TC-3 chambers. They have been designed to adapt to the most different types of samples and loading conditions, so the optimal grip conditions are obtained for plane sheet-shaped, rod-shapedorcylinder-shaped substrates.Click on image to enlargeMultiple parallel experimentsUp to three chambers can be actuated simultaneously in the TC-3 bioreactor. The loading conditions will be shared by all the chambers, whereas different culture conditions regarding the culture media may be established for each chamber.Click on image to enlargeHorizontal and vertical configurationsThe TC-3 can be configured both in horizontal and vertical configurations depending of the type of experiment you want to carry out. Air-liquid interfaces can be obtained with the horizontal configuration for cultures on sheet-like substrates, whereas the vertical configuration is more suitable for tests in which the sample is subjected to compssion like, e.g., cultures on cylinder-shaped scaffolds.Click on image to enlargeControl SoftwareThe TC-3 is computer operated by a simple control software that allows the user to create complex loading profiles to be applied on the culture samples. All the accessory configuration parameters have been removed from the interface, leaving only those which are of interest to be manipulated by the user.A graphical interface allows the user to easyly control and check the status of the system without the need of learning complex control softwares with a multitude of configuration parameters.